Case Western Reserve University dental researchers have found a less invasive way to extract single rare immune cells from the mouth to study how the mouth’s natural defenses ward off infection and inflammation.
By isolating some specialized immune cells (white blood cells known as “leukocytes”) to study how they fight diseases in the mouth – or reject foreign tissues, such as in failed organ transplants – researchers hope to learn more about treating and preventing such health issues as oral cancers, cardiovascular disease, AIDS and other infectious diseases.
To this point, researchers have had to rely on studying and growing immune cells from blood. Studying tissue immune cells allows researchers to learn how they function at the site of infection.
The role of adaptive immune cells in the stomach and intestines is more widely known, yet the role of similar cells in the mouth is unclear. There are no reliable methods to extract immune cells from mouth, which are more accessible and easier to extract than harder-to-reach tissues in the stomach and intestines.
But, until now, immune cells removed from the mouth couldn’t be isolated with enough viability or grown to study their activities, Pushpa Pandiyan, assistant professor of biological sciences at the dental school, explained.
The new method, developed by Pandiyan, the study’s lead author, is described in Biological Procedures article, “Isolation of T cells from mouse oral tissues.”
Pandiyan, who studies oral diseases associated with HIV, found no reliable method existed to isolate and keep a single cell from the tongue, gums and palate alive long enough to study.
Pandiyan and her team developed a way to do so successfully. The researchers reported that more than 94 percent of the isolated cells lived long enough to study.
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